File:Long-term tracking of a nuclear red stained chicken embryo.ogv

From Wikimedia Commons, the free media repository
Jump to navigation Jump to search

Original file ‎(Ogg Theora video file, length 10 s, 248 × 718 pixels, 3.9 Mbps, file size: 4.63 MB)

Captions

Captions

Add a one-line explanation of what this file represents

Summary[edit]

Description

Video 3. Long-term tracking of a nuclear red stained embryo.
Time-lapse movie of dorsal maximum z-projections of epiblast cells labeled with nuclear red showing their tracks from stage 5HH to 5 somites during PS regression. Colors indicate the z-position from magenta (dorsal) to yellow (ventral). t = 4 min between frames, z-sectioning = 4 μm. 20× objective, LSM 780. NMP: neuromesodermal progenitors; PMP: presomitic mesoderm progenitors; LPP: lateral plate progenitors. PS = primitive streak; NT: neural tube.

The nuclear red solution was prepared from the NucRed Live 647 ReadyProbes Reagent (Thermo Fisher) and diluted in PBS 1X as indicated by the manufacturer's experimental procedures. Sparse nuclear labeling of the dorsal epiblast was performed in ovo by injecting the nuclear red solution between the epiblast and the vitelline membrane at the PS level. The solution was left for 15 min to perform the long-term epiblast tracking and 30 min for monitoring ingression. The embryos were then dissected, rinsed in PBS, and mounted on paper filter for EC culture to perform live imaging from the dorsal side for the long-term epiblast tracking and from the ventral side for measuring cell ingression.
Date
Source

https://static-movie-usa.glencoesoftware.com/mp4/10.7554/787/de3d677f153809f96be901302a6b69b1154d71ed/elife-64819-video3.mp4 https://doi.org/10.7554/eLife.64819 Dynamics of primitive streak regression controls the fate of neuromesodermal progenitors in the chicken embryo

eLife 10:e64819.
Author Charlene Guillot Yannis Djeffal Arthur Michaut Brian Rabe Olivier Pourquié
This file, which was originally posted to an external website, has not yet been reviewed by an administrator or reviewer to confirm that the above license is valid. See Category:License review needed for further instructions.

Copyright

© 2021, Guillot et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Licensing[edit]

w:en:Creative Commons
attribution
This file is licensed under the Creative Commons Attribution 4.0 International license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current20:12, 2 May 202410 s, 248 × 718 (4.63 MB)Rasbak (talk | contribs){{Information |description= Video 3. Long-term tracking of a nuclear red stained embryo.<br> Time-lapse movie of dorsal maximum z-projections of epiblast cells labeled with nuclear red showing their tracks from stage 5HH to 5 somites during PS regression. Colors indicate the z-position from magenta (dorsal) to yellow (ventral). t = 4 min between frames, z-sectioning = 4 μm. 20× objective, LSM 780. NMP: neuromesodermal progenitors; PMP: presomitic mesoderm progenitors; LPP: lateral plate proge...

You cannot overwrite this file.

There are no pages that use this file.

Transcode status

Update transcode status
Format Bitrate Download Status Encode time
VP9 480P 908 kbps Completed 20:14, 2 May 2024 51 s
Streaming 480p (VP9) 947 kbps Completed 20:13, 2 May 2024 36 s
VP9 360P 544 kbps Completed 20:13, 2 May 2024 27 s
Streaming 360p (VP9) 562 kbps Completed 20:13, 2 May 2024 18 s
VP9 240P 231 kbps Completed 20:13, 2 May 2024 11 s
Streaming 240p (VP9) 231 kbps Completed 20:12, 2 May 2024 7.0 s
WebM 360P 516 kbps Completed 20:13, 2 May 2024 3.0 s
Streaming 144p (MJPEG) 1.13 Mbps Completed 20:12, 2 May 2024 2.0 s

File usage on other wikis

The following other wikis use this file:

Metadata

This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.

Retrieved from "https://commons.wikimedia.org/w/index.php?title=File:Long-term_tracking_of_a_nuclear_red_stained_chicken_embryo.ogv&oldid=873344172"