File:Human-RAD18-Interacts-with-Ubiquitylated-Chromatin-Components-and-Facilitates-RAD9-Recruitment-to-pone.0023155.s007.ogv
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Human-RAD18-Interacts-with-Ubiquitylated-Chromatin-Components-and-Facilitates-RAD9-Recruitment-to-pone.0023155.s007.ogv (Ogg Theora video file, length 10 s, 194 × 194 pixels, 30 kbps, file size: 39 KB)
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[edit]DescriptionHuman-RAD18-Interacts-with-Ubiquitylated-Chromatin-Components-and-Facilitates-RAD9-Recruitment-to-pone.0023155.s007.ogv |
English: Analyses of mutant RAD18 localization during M, G1 and S phase. Time lapse images of nuclei expressing YFP-RAD18 carrying a mutation in its Zinc finger (D221A). Images were captured every 30 min for 27 hours, a single nucleus was selected, aligned, and 5 frames of the images are shown per second. Endogenous RAD18 was downregulated by siRNA. Analyses began in G2 phase and the cell went through, M, G1, and most likely S. The cell cycle phases of mutant YFP-D221A were inferred from the time passed since the previous M-phase or the time span until the next M-phase, and the duration of G1, S, G2 and M phase in wild type cells. The growth rates of wild type and mutant cells were not different (data not shown). |
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Source | Movie S3 from Inagaki A, Sleddens-Linkels E, van Cappellen W, Hibbert R, Sixma T, Hoeijmakers J, Grootegoed J, Baarends W (2011). "Human RAD18 Interacts with Ubiquitylated Chromatin Components and Facilitates RAD9 Recruitment to DNA Double Strand Breaks". PLOS ONE. DOI:10.1371/journal.pone.0023155. PMID 21858012. PMC: 3157352. | ||
Author | Inagaki A, Sleddens-Linkels E, van Cappellen W, Hibbert R, Sixma T, Hoeijmakers J, Grootegoed J, Baarends W | ||
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current | 12:11, 18 November 2012 | 10 s, 194 × 194 (39 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Inagaki A, Sleddens-Linkels E, van Cappellen W, Hibbert R, Sixma T, Hoeijmakers J, Grootegoed J, Baarends W |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Analyses of mutant RAD18 localization during M, G1 and S phase. Time lapse images of nuclei expressing YFP-RAD18 carrying a mutation in its Zinc finger (D221A). Images were captured every 30 min for 27 hours, a single nucleus was selected, aligned, and 5 frames of the images are shown per second. Endogenous RAD18 was downregulated by siRNA. Analyses began in G2 phase and the cell went through, M, G1, and most likely S. The cell cycle phases of mutant YFP-D221A were inferred from the time passed since the previous M-phase or the time span until the next M-phase, and the duration of G1, S, G2 and M phase in wild type cells. The growth rates of wild type and mutant cells were not different (data not shown). |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011 |
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2011
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Categories:
- Videos of cell nucleus in HeLa cells
- Histone modification
- Videos of nucleic acids
- Videos of DNA repair
- Videos of cell cycle
- Chromatin
- Double-stranded DNA breaks
- DNA-binding proteins
- Fibroblasts
- Videos of histones
- Luminescent proteins
- Protein binding
- RNA interference
- Reverse transcriptase polymerase chain reaction
- Two-hybrid system techniques
- Ubiquitin
- Ultraviolet light
- Zinc fingers
- Media from PLOS ONE