File:High-Throughput-Ratio-Imaging-to-Profile-Caspase-Activity-Potential-Application-in-Multiparameter-pone.0020114.s002.ogv
High-Throughput-Ratio-Imaging-to-Profile-Caspase-Activity-Potential-Application-in-Multiparameter-pone.0020114.s002.ogv (Ogg Theora video file, length 3.9 s, 502 × 501 pixels, 8.03 Mbps, file size: 3.7 MB)
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[edit]DescriptionHigh-Throughput-Ratio-Imaging-to-Profile-Caspase-Activity-Potential-Application-in-Multiparameter-pone.0020114.s002.ogv |
English: Simultaneous visualization of TMRM loss and caspase activation. MCF7 SCAT3 cells stained with MMP indicator dye TMRM was treated with Actinomycin D and imaged every 10 minutes after drug addition. Imaging conditions are same as mentioned in Supplementary Movie S1. The frame rate is 30 frames per second. |
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Source | Movie S2 from Joseph J, Seervi M, Sobhan P, Retnabai S (2011). "High Throughput Ratio Imaging to Profile Caspase Activity: Potential Application in Multiparameter High Content Apoptosis Analysis and Drug Screening". PLOS ONE. DOI:10.1371/journal.pone.0020114. PMID 21637712. PMC: 3103529. | ||
Author | Joseph J, Seervi M, Sobhan P, Retnabai S | ||
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current | 15:37, 31 October 2012 | 3.9 s, 502 × 501 (3.7 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Joseph J, Seervi M, Sobhan P, Retnabai S |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Simultaneous visualization of TMRM loss and caspase activation. MCF7 SCAT3 cells stained with MMP indicator dye TMRM was treated with Actinomycin D and imaged every 10 minutes after drug addition. Imaging conditions are same as mentioned in Supplementary Movie S1. The frame rate is 30 frames per second. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011 |
- Antineoplastic agents
- Apoptosis
- Caspase 9
- Caspase catalytic domain
- Tumor cell line
- Chromatin
- Neoplasm drug resistance
- Antitumor drug screening assays
- Fluorescence resonance energy transfer
- High-throughput screening assays
- Three-dimensional imaging videos
- Mitochondrial membrane potentials
- Molecular probe techniques
- Proteases
- Media from PLOS ONE
- MCF-7 cells