File:Whole-organ thymus perfusion and decellularisation.webp
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DescriptionWhole-organ thymus perfusion and decellularisation.webp |
English: "a Gross appearance of cannulated rat thymi before (upper panels) and after (lower panels) decellularisation. A 24G cannula is inserted into the carotid artery and used to perfuse the organ with detergent and enzymatic solutions. Asterisks indicate extra-thymic tissues that allowed connection between thymic tissue and cannula through the large blood vessels (n = 120). Scale bar, 2 mm. b Micro-CT images of cannulated rat thymus showing extra-thymic tissues, large blood vessels and the 24G cannula entering the artery. Iodine contrast shows clear demarcation between cortex (C, bright) and medullary (M) areas; blood vessels (asterisks) are represented by very bright areas between and inside the parenchyma (n = 3). Scale bar, 1.5 mm. c Micro-CT 3D image of whole rat thymus cannulated where iodine contrast shows vasculature tree (segmented in red, n = 2). Scale bar, 1.2 mm. d Masson’s trichrome stain of a fresh rat thymus lobe staining in red keratins, in blue collagen and in pink cytoplasm. C cortex, M medulla (n = 3 thymi). Scale bar, 250 μm. e Haematoxylin & Eosin (H&E) stain of a fresh rat thymus. C cortex, M medulla (n = 3 thymi). Scale bar, 500 μm. f Micro-CT image of cannulated rat thymus showing the whole 2 lobes in 3D. Scale bar, 1.2 mm. g Micro-CT image of a cannulated rat thymus injected with Microfil® and thresholded to demonstrate perfusion of both thymic lobes. Scale bar, 1.2 mm. h Masson’s trichrome stain of a paraffin section of a decellularised rat thymus scaffold demonstrating collagen fibres (blue) and absence of keratins, muscle fibres and cell cytoplasm (n = 3 scaffolds). Scale bar, 250 μm. i H&E of a decellularised thymus scaffold showing intact thymic lobule ECM and preservation of both large and small vasculature wall (n = 4 scaffolds). Scale bar, 500 μm." |
Date | |
Source | https://www.nature.com/articles/s41467-020-20082-7 |
Author | Authors of the study: Sara Campinoti, Asllan Gjinovci, Roberta Ragazzini, Luca Zanieri, Linda Ariza-McNaughton, Marco Catucci, Stefan Boeing, Jong-Eun Park, John C. Hutchinson, Miguel Muñoz-Ruiz, Pierluigi G. Manti, Gianluca Vozza, Carlo E. Villa, Demetra-Ellie Phylactopoulos, Constance Maurer, Giuseppe Testa, Hans J. Stauss, Sarah A. Teichmann, Neil J. Sebire, Adrian C. Hayday, Dominique Bonnet & Paola Bonfanti |
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 15:28, 28 January 2021 | 1,998 × 1,779 (652 KB) | Prototyperspective (talk | contribs) | Uploaded a work by Authors of the study: Sara Campinoti, Asllan Gjinovci, Roberta Ragazzini, Luca Zanieri, Linda Ariza-McNaughton, Marco Catucci, Stefan Boeing, Jong-Eun Park, John C. Hutchinson, Miguel Muñoz-Ruiz, Pierluigi G. Manti, Gianluca Vozza, Carlo E. Villa, Demetra-Ellie Phylactopoulos, Constance Maurer, Giuseppe Testa, Hans J. Stauss, Sarah A. Teichmann, Neil J. Sebire, Adrian C. Hayday, Dominique Bonnet & Paola Bonfanti from https://www.nature.com/articles/s41467-020-20082-7 with U... |
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