File:Quantification of the number of epiblast cells electroporated chicken embryo.jpg

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Figure 2—figure supplement 1—source data 1 Number of electroporated cells after one or two pulses.—Quantification of the number of epiblast cells electroporated.

(A) Brightfield (left) and fluorescence (right) images of a stage 5HH chicken embryo 4 hr after one pulse of electroporation on each side of the anterior primitive streak (PS) with an H2B-RFP plasmid. The electroporated areas are within the orange box. (B) Higher magnification of the orange box shown in (A). Fluorescent nuclei in electroporated cells are shown in yellow, and the electroporated areas are circled in red. (C) Violin plot showing the quantification of the number of fluorescent nuclei 4 hr after one pulse or two successive pulses of electroporation at the same location (n = 4 embryos).
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https://elifesciences.org/articles/64819#fig2s1 https://doi.org/10.7554/eLife.64819 Dynamics of primitive streak regression controls the fate of neuromesodermal progenitors in the chicken embryo

eLife 10:e64819.
Author Charlene Guillot Yannis Djeffal Arthur Michaut Brian Rabe Olivier Pourquié
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© 2021, Guillot et al.

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current21:13, 1 May 2024Thumbnail for version as of 21:13, 1 May 2024730 × 1,687 (273 KB)Rasbak (talk | contribs)== {{int:filedesc}} == {{Information |description=Figure 2—figure supplement 1—source data 1 Number of electroporated cells after one or two pulses.—Quantification of the number of epiblast cells electroporated.<br> (A) Brightfield (left) and fluorescence (right) images of a stage 5HH chicken embryo 4 hr after one pulse of electroporation on each side of the anterior primitive streak (PS) with an H2B-RFP plasmid. The electroporated areas are within the orange box. (B) Higher magnification of...

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