File:Number of TSOX2 double-positive cells during early chicken stages embryo.jpg

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Figure 1—figure supplement 1—source data 1
Onset of SOX2/T expression cells in chicken.

(A–C) Representative maximum z-projections of confocal images of T and SOX2 whole-mount immunohistochemistry from stage 4 to stage 5HH. (D–F) Representative line profile intensities showing the quantification of the fluorescence intensity of the SOX2 (red) and T (green) staining along the anteroposterior axis of the embryos (y axis). 0 marks the anterior neural plate. Orange arrowheads: position of Hensen’s node representative of the anterior border of the primitive streak (PS). (G) Maximum z-projection of embryos stained in whole mount with SOX2/T antibodies showing the localization and the number of the SOX2/T double-positive cells (in yellow) in the anterior PS region. Within each color channel, we threshold the cells expressing T and SOX2 in the nucleus. The double-positive cells are identified by creating a new image with the thresholded image such as only cells expressing T and SOX2 are visible and quantified. Single positive cells are not shown. (H) Quantification of the number of SOX2/T double-positive cells between stage 4 and 5HH. Dorsal views, anterior to the top. Asterisk marks the tip of PS. White arrowheads: Hensen’s node (n = 18; seven embryos at stage 4HH, four at stage 4+HH, seven at stage 5HH). Scale bar: 100 µm.
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https://elifesciences.org/articles/64819#fig1s1 https://doi.org/10.7554/eLife.64819 Dynamics of primitive streak regression controls the fate of neuromesodermal progenitors in the chicken embryo

eLife 10:e64819.
Author Charlene Guillot Yannis Djeffal Arthur Michaut Brian Rabe Olivier Pourquié
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© 2021, Guillot et al.

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current20:59, 1 May 2024Thumbnail for version as of 20:59, 1 May 20242,007 × 2,502 (828 KB)Rasbak (talk | contribs){{Information |description= Figure 1—figure supplement 1—source data 1<br>Onset of SOX2/T expression cells in chicken.<br> (A–C) Representative maximum z-projections of confocal images of T and SOX2 whole-mount immunohistochemistry from stage 4 to stage 5HH. (D–F) Representative line profile intensities showing the quantification of the fluorescence intensity of the SOX2 (red) and T (green) staining along the anteroposterior axis of the embryos (y axis). 0 marks the anterior neural plate. Ora...

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