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English: Membrane constriction and reorganization of the host S-layer. (A, panel i) Three slices through tomograms showing Sulfolobus spindle-shaped virus 1 (SSV1) virions attached to the host cell surface. Scale bars, 20 nm. (ii and iii) Aligned and averaged (bold line) 2D contours of 7 virions (ii) and their budding necks (iii). Dw, average width; Dl, average length; Dn, neck diameter. (iv) Virion budding neck diameters, plotted against the aspect ratio of the virions. (B) Slices through tomograms of Sulfolobus shibatae, showing a semicrystalline S-layer (i) and compact S-layers (iv). S-layer thickness and the membrane (M) are indicated. (ii and v) Top views (left) and power spectra (right) of semicrystalline (ii) and compact (v) S-layers. (iii) Relative distribution of S-layer types at 0 (n = 195), 12 (n = 386), and 24 (n = 122) hpi. Only cells with the electron-dense cytoplasm were considered in calculating the S-layer phenotypes. (vi) Slice through a tomogram showing the S-layer rupture (arrows) during virion release. Scale bars, 50 nm (i and iv), 20 nm (ii and v), and 50 nm (vi).