File:Cilia are present and functional in the node of 4HH-8HH talpid3 chickens.jpg
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| Description | Figure 3. Cilia are present and functional in the node of 4HH-8HH talpid3 chickens. (a–f). Stage 4HH wildtype and talpid3 embryos underwent section immunocytochemistry for acetylated tubulin (ciliary axoneme, green) and γ tubulin (basal body, red) to identify cilia (white arrows). Short cilia were identified in the mesoderm of the nodes of both genotypes (b, e, arrows), while longer cilia were identified in greater abundance, in the epiblast (epi) and hypoblast (hypo) peripheral to the node (c, f, arrows. Arrowheads indicate telophase bridges which look similar to cilia but are not associated with a basal body). (g–j). Immunocytochemistry was carried out on 8HH embryos for acetylated tubulin to identify cilia (red arrows; blue arrow denotes telophase bridge). Cilia were identified in both nodal (g, i) and nonnodal tissues (h, j) in both wildtype (g, h) and talpid3 (i, j) embryos at 8HH. (k) qPCR show no significant difference between expression of PTCH1 in wildtype and talpid3 embryos (P = 0.84). PG-Primitive groove. (a–f) Magnifications comparable: a, d-magnification 10×, b-magnification 50×, c, e, f-magnification 100×. Magnification of g–j the same (not comparable to a–f). |
| Date | |
| Source | https://doi.org/10.1002/dvg.22775 (2014), The chicken left right organizer has nonmotile cilia which are lost in a stage-dependent manner in the talpid3 ciliopathy. genesis, 52: 600-613. |
| Author | Stephen, L.A., Johnson, E.J., Davis, G.M., McTeir, L., Pinkham, J., Jaberi, N. and Davey, M.G. |
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| current | 17:36, 5 May 2024 | | 1,063 × 1,402 (582 KB) | Rasbak (talk | contribs) | {{Information |description=Figure 3. Cilia are present and functional in the node of 4HH-8HH talpid3 chickens. (a–f). Stage 4HH wildtype and talpid3 embryos underwent section immunocytochemistry for acetylated tubulin (ciliary axoneme, green) and γ tubulin (basal body, red) to identify cilia (white arrows). Short cilia were identified in the mesoderm of the nodes of both genotypes (b, e, arrows), while longer cilia were identified in greater abundance, in the epiblast (epi) and hypoblast (hyp... |
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