File:An anterior to posterior gradient of proliferation counteracts ingression in the anterior primitive streak (PS) epiblast chicken embryo.jpg

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Figure 7. An anterior to posterior gradient of proliferation counteracts ingression in the anterior primitive streak (PS) epiblast.

(A) Quantification of the number of SOX2/T double-positive cells in chicken embryos from stage 4HH to 35-somite (n = 38 embryos). (B) Snapshots of the posterior region of chicken embryos from stage 5HH to 20-somite stained in whole-mount with an anti-phosphorylated histone H3 (pH3) antibody. (C) Quantifications of the mitotic index along the PS in the boxes shown in (B). Orange box: 250 µm from node; green box: 750 µm from node; blue box: 1200 µm from node (n = 13 embryos). Unpaired t-test; **p=0.0017 and 0.0022; *p=0.0187, p=0.0278. (D) Quantification of the number of dividing cells in H2B-Cherry transgenic quails at stage 4+/5HH in the anterior and posterior PS (n = 6). Paired t-test; ***p=0.0001. (E) Tracks and (F) quantification of trajectories of the neuromesodermal progenitor (NMP) (gold) and lateral plate progenitor (LPP) (gray) cells during PS regression (n = 159 cells, 80 posterior, 79 anterior in seven embryos). (G) Quantification of the time interval between two rounds of division in cells of the NMP region measured in time-lapse movies (n = 12 inter-division events in four embryos). (B, E) Dorsal views, anterior to the top. Arrowhead shows Hensen’s node position. Scale bar: 100 µm.
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https://iiif.elifesciences.org/lax/64819%2Felife-64819-fig7-v2.tif/full/,1500/0/default.jpg https://doi.org/10.7554/eLife.64819 Dynamics of primitive streak regression controls the fate of neuromesodermal progenitors in the chicken embryo

eLife 10:e64819.
Author Charlene Guillot Yannis Djeffal Arthur Michaut Brian Rabe Olivier Pourquié
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© 2021, Guillot et al.

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current00:52, 3 May 2024Thumbnail for version as of 00:52, 3 May 20241,268 × 1,637 (353 KB)Rasbak (talk | contribs){{Information |description= Figure 7. An anterior to posterior gradient of proliferation counteracts ingression in the anterior primitive streak (PS) epiblast.<br> (A) Quantification of the number of SOX2/T double-positive cells in chicken embryos from stage 4HH to 35-somite (n = 38 embryos). (B) Snapshots of the posterior region of chicken embryos from stage 5HH to 20-somite stained in whole-mount with an anti-phosphorylated histone H3 (pH3) antibody. (C) Quantifications of the mitotic index...

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